Determination of Caries Lesion Activity: Reflection and Roughness for Characterization of Caries Progression

Used by permission. © Operative Dentistry, Inc. Transmission or reproduction of protected items beyond that allowed by fair use requires the written permission of Operative Dentistry, Inc.Caries lesion progression is difficult to determine with visual and tactile examinations. The hypothesis of this study was that reflection and roughness measurements could determine caries progression. Ground/polished sound human enamel specimens were analyzed at baseline (sound) and after two four-day demineralization periods for reflection using optical reflectometry (ORef) and for roughness using optical surface profilometry (SPro). Specimens were demineralized using a microbial–Streptococcus mutans aries model. Comparisons among the periods for ORef and SPro were performed using repeated measures analysis of variance. Two-sample t-tests were used for differences in transverse microradiography. The integrated mineral loss and depth of the four-day demineralization period were significantly smaller than those for the eight-day demineralization period (p<0.01). With increased demineralization time, reflection was significantly decreased and roughness was significantly increased (p<0.01). Correlation between ORef and SPro was moderate (r=−0.63). Both reflection and roughness can be characterized for nondestructive longitudinal assessment of caries lesion progression

Present status of fish culture in daulatabad talaw(amrai talaw) dist- Aurangabad (M.S.), India

Present study is give to fish culture status, production and development of talaw. An attempt has been made to involve present status for fishery culture improvement in the management and commercial production of the fishes. It controlled by private agency

Inference with Imperfect Randomization: The Case of the Perry Preschool Program

This paper considers the problem of making inferences about the effects of a program on multiple outcomes when the assignment of treatment status is imperfectly randomized. By imperfect randomization we mean that treatment status is reassigned after an initial randomization on the basis of characteristics that may be observed or unobserved by the analyst. We develop a partial identification approach to this problem that makes use of information limiting the extent to which randomization is imperfect to show that it is still possible to make nontrivial inferences about the effects of the program in such settings. We consider a family of null hypotheses in which each null hypothesis specifies that the program has no effect on one of several outcomes of interest. Under weak assumptions, we construct a procedure for testing this family of null hypotheses in a way that controls the familywise error rate – the probability of even one false rejection – infinite samples. We develop our methodology in the context of a reanalysis of the HighScope Perry Preschool program. We find statistically significant effects of the program on a number of different outcomes of interest, including outcomes related to criminal activity for males and females, even after accounting for the imperfectness of the randomization and the multiplicity of null hypotheses.multiple testing, multiple outcomes, randomized trial, randomization tests, imperfect randomization, Perry Preschool Program, program evaluation, familywise error rate, exact inference, partial identification

Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly

AbstractThe gut is the most extensive, interactive, and complex interface between the human host and the environment and therefore a critical site of immunological activity. Non-invasive methods to assess the host response in this organ are currently lacking. Feces are the available analyte which have been in proximity to the gut tissue.We applied a method of concentrating host transcripts from fecal specimens using a existing bead-based affinity separation method for nucleic acids and quantified transcripts using droplet digital PCR (ddPCR) to determine the copy numbers of a variety of key transcripts in the gut immune system. ddPCR compartmentalizes the reaction in a small aqueous droplet suspended in oil, and counts droplets as either fluorescent or non-fluorescent. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used to normalize transcript concentration.This method was applied to 799 fecal samples from rural Malawian children, and over 20,000 transcript concentrations were quantified. Host mRNA was detected in >99% samples, a threshold for target detection was established at an average expression of 0.02 copies target/GAPDH, above which correlation coefficient between duplicate measurements is >0.95. Quantities of transcript detected using ddPCR were greater than standard qPCR. Fecal sample preservation at the time of collection did not require immediate freezing or the addition of buffers or enzymes. Measurements of transcripts encoding immunoactive proteins correlated with a measure of gut inflammation in the study children, thereby substantiating their relevance. This method allows investigators to interrogate gene expression in the gut

Studies on some free living protozoan from Salim Ali lake. Aurangabad

Protozoa are one-celled animals found worldwide in most habitats. Most species are free living, but all higher animals are infected with one or more species of protozoa. Infections range from asymptomatic to life threatening, depending on the species and strain of the parasite and the resistance of the host. Present study deals with the free living protozoa inhabiting fresh water bodies. The factor which influence their distribution and population in a given water bodies temperature, light, pH, chemical composition, acidity, and amount of food present in water and degree of adaptability of the individual protozoa to various environmental changes.&nbsp

A Comprehensive Review of Dynamic Voltage Restorer

Power quality is a major concern at the present time. Become important, especially with the introduction of advanced performance devices that are extremely sensitive to the quality of food. Power quality problem is the event that takes place before a non-standard voltage, current, or frequency, leading to end-use hardware failure. One of the main issues addressed here is sagging power. To resolve this issue, one must use custom power devices. One of these devices is the Dynamic Voltage Restorer (DVR), a modern custom power device used in the most efficient power distribution networks. Call has a lower cost, smaller size, rapid dynamic response and disorder. This paper examines a review of the DVR technology and the various applications that have been proposed in the literature